I am a lab technician, and I work with samples that contain (or may contain) SARS-CoV-2, the virus that causes COVID-19. What level of biosafety precautions should I follow?
Frequently Asked Questions
Question: I am a lab technician, and I work with samples that contain (or may contain) SARS-CoV-2, the virus that causes COVID-19. What level of biosafety precautions should I follow?
Answer:
The Centers for Disease Control and Prevention (CDC) describes the appropriate biosafety level (BSL) protection for various types of tasks in its Interim Guidelines for Collecting, Handling, and Testing Clinical Specimens for COVID-19 and Interim Laboratory Biosafety Guidelines for Handling and Processing Specimens Associated with COVID-19.
In general, CDC recommends that for:
- Routine diagnostic testing of clinical specimens (e.g., processing initial samples, applying stains to fixed smears, and pathologic examination of inactivated tissues), work should be performed at BSL-2;
- Environmental specimen testing that involves virus concentration procedures (e.g., sewage surveillance testing), work should be performed at BSL-2 with BSL-3 precautions, including respiratory protection and a designated area for putting on and taking off personal protective equipment;
- Virus isolation in cell cultures, work should be performed at BSL-3;
- Environmental specimen testing that concentrates virus, work should be performed at BSL-2 with BSL-3 precautions.
For viral testing of specimens conducted outside of a BSL-2 laboratory, such as rapid respiratory testing performed at the point of care, use standard precautions to provide a barrier between the specimen and personnel during specimen manipulation. For more information on specimen collection, handling, and testing, refer to the CDC's Interim Guidelines for Collecting, Handling, and Testing Clinical Specimens for COVID-19.
Aerosol-generating procedures and work with concentrated virus should always be performed in an appropriately maintained and certified biosafety cabinet.
OSHA also discusses laboratory biosafety on its COVID-19 Safety and Health Topics page. This guidance is generally consistent with CDC's Biosafety in Microbiological and Biomedical Laboratories (BMBL), 5th Edition.