1. OSHA Occupational Chemical Database
  2. METHOTREXATE
Label abbreviations descriptions

METHOTREXATE

Chemical Identification
Chemical Identification
CAS # 59-05-2
Formula

C₂₀H₂₂N₈O₅
Synonyms

amethopterin; 4-amino-l0-methylfolic acid; 1-glutamic acid, N-(4-(((2,4-diamino-6-pteridinyl)methyl)methylamino))benzoyl; glutamic acid, N-(p-(((2,4-diamino-6-pteridinyl)methyl)methylamino)benzoyl)-, L; methopterin; amethopterine; HDMTX; MTX; L-N-(p-(((2,4-diamino-6-pteridinyl)methyl)methylamino)benzoyl) glutamic acid
Physical Properties
Physical Properties
Physical description Bright yellow-orange, odorless, crystalline powder.
Boiling point Molecular weight 454.46
Freezing point/melting point 356-399°F Vapor pressure
Flash point Vapor density
Specific gravity Ionization potential
Lower explosive limit (LEL) Upper explosive limit (UEL)
NFPA health rating NFPA fire rating
NFPA reactivity rating NFPA special instruction
Vapor hazard ratio (VHR)
Historical exceedance percentage
Target organs
Monitoring Methods Used by OSHA
Monitoring Methods Used by OSHA
Analyte code (IMIS no.) M106
Sampling group
Sampler/Sampling media OSHA versatile sampler (OVS-2) - 13 mm XAD-2 tube (270/140 mg) with GFF enclosed [SKC 226-30-16]
Sampling time*

120 min
Sampling volume (TWA)*

120 L
Sampling flow rate (TWA)*

1 L/min
Sampling volume (STEL/Peak/C)*
 

 
Sampling flow rate (STEL/Peak/C)*
Analytical method instruments HPLC
Method reference OSHA PV2146 (partially validated)
Notes
 

 
Special requirements
 

 

* All sampling instructions above are recommended guidelines for OSHA Compliance Safety and Health Officers (CSHOs), please see the corresponding OSHA method reference for complete details.

Wipe Method
Wipe Method
Sampler/Sampling media
 

 
Bulk Method
Bulk Method
 

 
On-Site Screening Techniques
On-Site Screening Techniques
Device
Model/Type
Sampling information
(see manufacturer instructions)
Exposure Limits
Exposure Limits
OSHA PEL
8-hour TWA
(ST) STEL
(C) Ceiling
Peak
NIOSH REL
Up to 10-hour TWA
(ST) STEL
(C) Ceiling
ACGIH TLV©
8-hour TWA
(ST) STEL
(C) Ceiling
CAL/OSHA PEL
8-hour TWA
(ST) STEL
(C) Ceiling
Peak
PEL-TWA
 

 

 REL-TWA
 

 

 TLV-TWA
 

 

 PEL-TWA
 

 
PEL-STEL
 

 

 REL-STEL
 

 

 TLV-STEL
 

 

 PEL-STEL
 

 
PEL-C
 

 

 REL-C
 

 

 TLV-C
 

 

 PEL-C
 

 
Skin notation
 

NA

 Skin notation
 

NA

 Skin notation
 

NA

 Skin notation
 

NA
Notes:
 

Not established

 Notes:
 

Not established

 Notes:
 

Not established

 Notes:
 

Not established
Health factors:  See NIH-NLM PubChem. IDLH
Carcinogenic classifications: IARC-3 Notes:
 

 
AIHA emergency response planning guidelines - ERPG-1/ERPG-2/ERPG-3:
 

 
Additional Resources and Literature References
Additional Resources and Literature References

NOAA: CAMEO Chemicals - Methotrexate

NIOSH: Pocket Guide to Chemical Hazards - Methotrexate

Literature References

 
  • Bawle, E.V., Conard, J.V. and Weiss, L.: Adult and two children with fetal methotrexate syndrome. Teratology 57(2): 51-55, 1998.
  • Dastychová, E.: Allergic contact dermatitis in methotrexate manufacture. Contact Dermatitis 48(4): 226-227, 2003.
  • Deng, H., et al.: Investigating genetic damage in workers occupationally exposed to methotrexate using three genetic end-points. Mutagenesis 20(5): 351-357, 2005.
  • Izzedine, H., Launay-Vacher, V. Karie, S., Caramella, C., de Person, F. and Deray, G.: Is low-dose methotrexate nephrotoxic? Case report and review of the literature. Clin. Nephrol. 64(4): 315-319, 2005.
  • Kalantzis, A., Marshman, Z., Falconer, D.T., Morgan, P.R. and Odell E.W.: Oral effects of low-dose methotrexate treatment. Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod. 100(1): 52-62, 2005.
  • Lateef, O., Shakoor, N. and Balk, R.A.: Methotrexate pulmonary toxicity. Expert Opin. Drug Saf. 4(4): 723-730, 2005.
  • Pohanish, R.P. (editor): Methotrexate. In, Sittig's Handbook of Toxic and Hazardous Chemicals and Carcinogens, Fourth Ed., Vol. 2. Norwich, NY: Noyes Publications, William Andrew Publishing, 2002, pp. 1504-1505.
  • Sessink, P.J., Friemel, N.S., Anzion, R.B. and Bos, R.P.: Biological and environmental monitoring of occupational exposure of pharmaceutical plant workers to methotrexate. Int. Arch. Occup. Environ. Health 65(6): 401-403, 1994.

Last Updated Date : 01/05/2021