Method no.:	29
Matrix:	Air
Target concentration:	Halothane - 1 ppm (8.1 mg/m3) Enflurane - 1 ppm (7.6 mg/m3)
Procedure:	Two standard size charcoal tubes are connected in series for each sample. Samples are desorbed with carbon disulfide (CS2) and analyzed by gas chromatography (GC) using a flame ionization detector (FID).
Recommended air volume and sampling rate:	10 L at 0.1 L/min
Reliable quantitation limit:	Halothane - 0.023 ppm (0.19 mg/m3) Enflurane - 0.040 ppm (0.30 mg/m3)
Standard error of estimate at the target concentration:
(Figure 4.7.4.) (Figure 4.7.2.)	Halothane - 6.43% Enflurane - 8.01%
Status of method:	A sampling and analytical method which has been subjected to the established evaluation procedures of the Organic Methods Evaluation Branch.
Date: May 1981	Chemist: Mike Shulsky

1. General Discussion

1.1. Background

1.1.1. History

Halothane has been collected in glass sampling bulbs with subsequent GC analysis using an electron capture detector. (Ref. 5.1.) Charcoal passive dosimeters have been used for collection of both enflurane and halothane with GC/FID analysis (Ref. 5.2.).

Charcoal tubes were used in this evaluation because of their widespread use for other organics and their efficiency for collecting both analytes. Also, with charcoal high desorptions were obtained for both analytes. The analytical method is sensitive and analytical parameters can be adjusted to eliminate interferences.

NIOSH recommends a 1 h ceiling value of 2 ppm for both halothane and enflurane (Ref. 5.4.). Since ceiling values in the Federal Register are usually twice the 8-h TWA PEL, a target concentration of 1 ppm was chosen.

1.1.2. Toxic effects (This section is for information only and should not be taken as the basis of OSHA policy).

There are conflicting reports in the literature dealing with the effects of exposure of humans to low levels of anesthetics. One study indicates that chronic low-level exposure of female operating room personnel to anesthetics in general can cause spontaneous abortion (Ref. 5.3.). Animal studies have shown that chronic halothane exposures of 50-150 ppm can cause liver and kidney damage (Ref. 5.4.). At sub-anesthetic levels, enflurane exposure produced early mortality and detrimental weight effects in mice although the same tests had no apparent effect on rats or guinea pigs (Ref. 5.5.).

1.1.3. Workplace exposure

Enflurane and halothane are two of the three most commonly used anesthetics. Occupational exposure may occur wherever anesthetics are used such as operating rooms, veterinarian hospitals, and dental offices. The number of people potentially exposed is estimated to be 215,000 (Ref. 5.4.).

1.1.4. Physical properties (Ref. 5.4. unless otherwise stated)

halothane
molecular weight:	197.4
boiling point:	50°C
specific gravity:	1.871 (Ref. 5.6.)
vapor pressure:	243 mm Hg (20°C)
synonyms:	2-bromo-2-chloro-1,1,1-trifluoroethane, fluothane
formula:	CHClBrCF3
enflurane
molecular weight:	184.5
boiling point:	56°C
specific gravity:	1.52
vapor pressure:	175 mm Hg (20°C)
synonyms:	2-chloro-1,1,2-trifluoroethyldifluoro-methylether, ethrane
formula:	CHFClCF2OCHF2

1.2. Limit defining parameters

1.2.1. Detection limit of the analytical procedure

The detection limit of the analytical procedure is 6.08 ng/injection for enflurane and 3.74 ng/injection for halothane. These are the amounts which give peaks about 5 times the baseline noise. (Section 4.1.1.)

1.2.2. Detection limit of the overall procedure

The detection limit for the overall procedure is 3.04 µg/sample (0.04 ppm or 0.3 mg/m³) for enflurane and 1.87 µg/sample (0.023 ppm or 0.19 mg/m³) for halothane. This is the amount of analyte spiked on a charcoal tube which allows recovery of an amount equal to the detection limit of the analytical procedure. (Section 4.1.2.)

1.2.3. Reliable quantitation limit

The reliable quantitation limit is 3.04 µg/sample (0.04 ppm or 0.3 mg/m³) for enflurane and 1.87 µg/sample (0.023 ppm or 0.19 mg/m³) for halothane. This is the smallest amount of analyte which can be quantitated within the requirements of a recovery of at least 75% and a precision of ±25% or better. (Section 4.2.)

---

The reliable quantitation limit and detection limits reported in this method are based on optimization of the instrument for the smallest possible amount of analyte. When the target concentration is exceptionally higher than these limits, they may not be attainable at the routine operating parameters.

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1.2.4. Sensitivity

The sensitivity of the analytical procedure over the concentration range representing 0.5 to 2 times the target concentration is 496 area counts/(µg/mL) for enflurane and 534 area counts/(µg/mL) for halothane. The sensitivity is determined by the slope of the calibration curves. (Section 4.4.) Sensitivity will vary somewhat with the particular instrument used for analysis and method of integration.

1.2.5. Recovery

The recovery of the analyte from the collection medium must be 75% or greater. The average recovery from spiked charcoal tubes over the range of 0.5 to 2 times the target concentration was essentially 100% for both enflurane and halothane. (Section 4.6.)

1.2.6. Precision (analytical method)

The pooled coefficients of variation obtained from replicate determinations of analytical standards at 0.5, 1, and 2 times the target concentration are 0.059 for enflurane and 0.029 for halothane. (Section 4.3.1.)

1.2.7. Precision (overall procedure)

The precision at the 95% confidence level for the 15-day storage test is ±15.6% and ±12.6% for enflurane and halothane, respectively. (Section 4.3.2.) These values include an additional ±5% for sampling error. The overall procedure must provide results at the target concentration that are ±25% or less at the 95% confidence level.

1.3. Advantages

1.3.1. The sampling apparatus is portable and easy to use.

1.3.2. No loss of analytes is observed after 2 weeks of storage.

1.3.3. The samples can be reanalyzed several times.

1.3.4. The analytical method is easy and convenient.

1.4. Disadvantages

1.4.1. The method has not been field tested.

1.4.2. The solvent used for desorption is toxic and flammable so it must be handled with care.

2. Sampling Procedure

2.1. Apparatus

2.1.1. A personal sampling pump which can be calibrated within ±5% of the 0.1 L/min flow rate.

2.1.2. Coconut shell charcoal tubes (glass tubes 7-cm length, 6-mm o.d., 4-mm i.d.) are used. They contain 100-mg and 50-mg portions of charcoal and the tube is heat sealed at both ends. For this evaluation, tubes from SKC lot 120 charcoal were used.

2.2. Reagents

None required

2.3. Technique

2.3.1. Immediately before sampling, break open the ends of the charcoal tubes and connect two charcoal tubes in series per sample. The small, red, plastic SKC cap with the closed end removed is convenient for this. Position the 50-mg portion of the tubes nearest the sampling pump. All tubes should be from the same lot of charcoal.

2.3.2. Properly label each pair of tubes to distinguish the front and backup tubes.

2.3.3. The tubes are connected to the sampling pump with a piece of flexible tubing. The tube nearest the pump should be labeled as the backup portion of the sample.

2.3.4. The tubes should be placed vertically to avoid channeling through the charcoal.

2.3.5. The exposed, broken end of the first charcoal tube should be enclosed in a tube holder to avoid injury to the worker.

2.3.6. Air being sampled should not pass through any tubing before entering the first charcoal tube.

2.3.7. After sampling, separate the pairs of tubes and seal each tube with plastic caps.

2.3.8. With each set of samples, submit at least one blank tube. The blank should be treated in the same manner as the samples (break ends, seal, transport) except no air is drawn through it.

2.3.9. Transport the samples and paperwork to the lab for analysis.

2.3.10. If bulk samples are to be submitted, they must be sent in a mailing package separate from any air samples being submitted. This is to avoid contamination of the air samples if the bulk should leak or get broken in transit.

2.4. Breakthrough

Studies to determine the 5% breakthrough values were done for enflurane and halothane simultaneously at twice the target concentration using an entire charcoal tube ("A" and "B" portion) with a flow of 0.1 L/min. The average breakthrough air volumes were 18.6 L for enflurane and 16.4 L for halothane. Average capacities of the tubes were 0.255 mg of enflurane and 0.252 mg for halothane. (Section 4.5.)

2.5. Desorption efficiency

The desorption efficiency was determined at several loadings of enflurane and halothane by liquid injection of CS₂ solutions of these analytes. The amount of analytes added corresponded to the detection limit of the analytical procedure, 0.5, 1, and 2 times the target concentration. The "A" and "B" portions of each tube were placed in one vial and desorbed with 1 mL of CS₂/internal standard solution. The desorption efficiencies for all loadings and both analytes were essentially 100%. (Section 4.6.) Desorption efficiencies may vary from one lot of charcoal to another.

2.6. Recommended air volume and sampling rate

The recommended air volume is 10 L at a flow rate of 0.1 L/min or lower.

2.7. Interferences

2.7.1. Charcoal will adsorb many organic vapors so organics being used in significant quantities near the sampling area should be listed as possible interferences.

2.7.2. High relative humidity and temperature will decrease the capacity of the charcoal for halothane and enflurane. If these conditions are encountered, it may be advisable to decrease the total volume of air sampled. The recommended 10-L air volume was based on sampling air at 22°C and 80% relative humidity.

2.8. Safety precautions

2.8.1. Wear eye protection when breaking the ends of the charcoal tubes.

2.8.2. Place the sampling apparatus on the employee so as not to interfere with the work being done.

2.8.3. Adequate precautions should be taken at all times to prevent injury from the broken ends of the charcoal tubes.

3. Analytical Method

3.1. Apparatus

3.1.1. Gas chromatograph (GC) equipped with a flame ionization detector.

3.1.2. A GC column is needed which is capable of separating carbon disulfide, enflurane, halothane, and if used, an internal standard compound. For this evaluation, a 20 ft stainless steel column packed with 10% Tergitol NP-35 on 80/100 Chrom P-AW DMCS was used.

3.1.3. A suitable method for peak area integration.

3.1.4. Small vials with Teflon-lined caps are needed for desorption of charcoal. Two-milliliter vials are preferable.

3.1.5. Microliter syringes; 2-µL and 10-µL

3.1.6. Pipettes of various sizes for desorption and dilution are needed. A solvent dispenser is convenient for sample desorption.

3.1.7. Volumetric flasks; 5-, 10-, 25-mL

3.2. Reagents

3.2.1. Carbon disulfide; reagent grade

3.2.2. Halothane; USP grade

3.2.3. Enflurane; USP grade

3.2.4. For this evaluation, benzene was used as an internal standard (1 µL/mL in CS₂). Since it may not be feasible to use benzene in other laboratories, several other compounds were tried but found to be unsatisfactory either because of interferences or considerable lengthening of analysis time. The compounds tried were hexane, cyclohexane, methyl cyclohexane, heptane, isooctane, octane, nonane, bromomethane, chloroform, methyl chloroform, Freon 113, trichloroethylene, and tetrachloroethylene. An external standard method may be used instead of an internal standard method.

3.2.5. GC grade nitrogen or helium, hydrogen and air

3.3. Standard preparation

Standards should be prepared at concentrations corresponding to 80.7 µg/mL for halothane and 75.5 µg/mL for enflurane. This is representative of samples taken at the target concentration assuming a 10-L air volume.

3.4. Sample preparation

3.4.1. Both sections of charcoal in the first tube are placed in one vial while both sections of the second tube (backup tube) are placed in a separate vial. One milliliter of desorbing solvent is added to each vial.

3.4.2. The vials are immediately capped and shaken intermittently over a 30-min period before analysis.

3.5. Analysis

3.5.1. Conditions for this evaluation

GC temperatures
oven:	70°C
injector:	150°C
detector:	150°C
gas flow rates
carrier (He):	25 mL/min.
hydrogen:	23 mL/min.
air:	250 mL/min.
retention times
enflurane:	12.61 min
halothane:	13.83 min.
benzene:	19.84 min
injection size:	2 µL
detector:	flame ionization
chromatogram:	Figure 4.8.

3.5.2. Peak areas are measured by a suitable technique.

3.5.3. A calibration method for our data system was prepared by setting the peak areas from the analytical standards equal to the corresponding air concentration in ppm assuming a 10-L air volume, using 1 mL for desorption.

Example:

Halothane standard:	86 µg/mL
Assume 10-L air volume

1-mL desorption volume: 99% desorption efficiency; 24.46 is molar volume of an ideal gas at 25°C and 760 mm Hg; 197.39 MW of Halothane

ppm = (86 µg/mL)(1 mL)(24.46)/(10 L)(197.39)(0.99)

ppm (Halothane) = 1.08

By calibrating the area of the analyte to its corresponding air concentration, the areas obtained from actual samples can be expressed in ppm assuming a 10-L air volume. The correction for these results to the actual air volume for a sample is discussed in Section 3.7.1.

3.5.4. Any samples above the target concentration must be confirmed by GC/MS or another suitable method. Retention time on one column is not considered proof of identity.

3.6. Interferences

3.6.1. Any compound which has the same retention time as halothane or enflurane will be an interference.

3.6.2. The solvent, CS₂, generally will contain impurities which may be significant at the analytical levels.

3.7. Calculations

3.7.1. The values obtained from the calibration method described in Section 3.5.3. will be in ppm based on a 10-L air volume. To convert this ppm value to a ppm value based on the actual air volume for each sample, the actual air volume is divided by 10 L and that quotient is divided into the ppm value from the calibration method.

Example:	1 ppm from calibration method
	12-L air volume

12 L 10 L

= 1.2

1 ppm 1.2

= 0.8 ppm

Reported result   =   0.8 ppm

3.7.2. If there is analyte present in the backup tube then the same air volume correction must be made. This corrected ppm value is added to that value from the front tube and the total is reported. If the corrected ppm value from the backup tube is 25% or more of the corrected value from the front tube, then a note should be placed on the analysis report sheets indicating that the particular sample should be considered saturated.

3.8. Safety precautions

3.8.1. Work in a hood when using solvents.

3.8.2. Keep volumetrics and vials containing solvents away from sources of high temperature such as GC injectors and detectors.

3.8.3. Avoid skin contact with all solvents.

3.8.4. Wear safety glasses at all times.

4. Backup Data

4.1. Detection limits

4.1.1. Analytical procedure

The detection limits were determined by injecting 2 µL of an analytical standard containing 3.04 µg/mL enflurane and 1.87 µg/mL halothane. The chromatographic peaks obtained for the analytes were approximately 5 times the baseline noise. Therefore, the detection limit of the analytical procedure is 6.08 ng/injection for enflurane and 3.74 ng/injection for halothane (Figure 4.1.1.).

4.1.2. Overall procedure

The detection limit of the overall procedure is 3.04 µg/sample (0.040 ppm or 0.30 mg/m³) for enflurane and 1.87 µg/sample (0.023 ppm or 0.10 mg/m³) for halothane. These are the amounts of respective analyte spiked on a charcoal tube which allow recovery of amounts equal to the respective detection limits of the analytical procedure (Table 4.6.1. and 4.6.2., Figure 4.6.1. and 4.6.2.). These limits assume the injection size (2 µL) of the analytical method.

4.2. Reliable quantitation limit

The reliable quantitation limit is 3.04 µg/sample (0.040 ppm or 0.3 mg/m³) for enflurane and 1.87 µg/sample (0.023 ppm or 0.19 mg/m³) for halothane. This is the smallest amount which can be spiked on a charcoal tube, which will give at least 75% recovery and a precision within ±25% at the 95% confidence level. The precision (1.96 SD) of the data listed in Tables 4.6.1 and 4.6.2. for the above concentrations is ±3% for halothane and ±2% for enflurane. This limit was determined assuming the injection size (2 µL) of the analytical method.

4.3. Precision data

4.3.1. Analytical method

Precision was determined by replicate injections of analytical standards prepared at concentrations equivalent to 0.5, 1, and 2 times the target concentration. The pooled coefficient of variation is 0.059 for enflurane and 0.029 for halothane.

Table 4.3.1.1.
Analytical Precision for Halothane

× target conc.	0.5×	1×	2×
area counts	12002	24316	45953
	11851	24070	47323
	11921	24047	45316
	11322	25971	46503
	12423	24196	45369
	12073	23356	45685
	11932	24326	46025
SD	359	873	770
CV	0.030	0.036	0.017
= 0.029

Table 4.3.1.2.
Analytical Precision of Enflurane

× target conc.	0.5×	1×	2×
area counts	7318	16791	33271
	7377	19266	33832
	7188	15538	32004
	7024	15989	33310
	7732	15776	33256
	7464	15096	31904
	7351	16409	32929
SD	242	1508	786
CV	0.033	0.092	0.030
= 0.059

4.3.2. Overall procedure

The precision for the overall procedure includes ±5% for sampling and any storage effects. This precision was found by plotting the storage data from Table 4.7.1., Figures 4.7.1. - 4.7.4. The precision for enflurane is ±16.9% (Figure 4.7.2.) and ±13.5% for halothane (Figure 4.7.4.).

4.4. Sensitivity

The sensitivity is taken as the slope of the calibration curves obtained by plotting data Tables 4.3.1.1. and 4.3.1.2. The sensitivity for enflurane is 496 area counts/(µg/mL) (Figure 4.4.1.) and 534 area counts/(µg/mL) for halothane (Figure 4.4.2.).

4.5. Sampler Capacity

Breakthrough is defined as the point during sampling when the analyte concentration in the air downstream from the charcoal tube is 5% of the concentration ahead of the tube. To determine the breakthrough point, a vapor generation system was used.

The atmosphere in the system was 2 ppm of halothane and enflurane, each, with approximately 80% relative humidity at 21°C. Sampling this atmosphere was performed using a charcoal tube containing both sections of charcoal.

Monitoring the downstream concentration of analytes was done by using a gas sampling valve (GSV) connected to a GC. The sample in the GSV was injected, at specified time intervals, into the GC column in order to separate the two analytes. An FID was used for detection.

Before a charcoal tube was put in line, a by-pass was used so the analytes' concentrations downstream would be the same as ahead of the tube. This allowed the monitoring of the analytes' concentrations in the system, so the 100% value could be determined. When the analytes' concentrations had stabilized, the air was diverted from the by-pass to the charcoal tube at the start of a gas valve sampling cycle; this is time zero. Since the air sampled at time zero had not passed through the charcoal tube, it's concentration of analytes was essentially 100%. Depending on the flow rate through the GSV, and the GC run time, the concentration of the analytes in the air sampled will drop to zero within the next few GSV cycles.

Four separate breakthrough determinations were performed by sampling the above mentioned atmosphere (2 ppm enflurane and halothane, 80% R.H.) at a flow rate of 0.1 L/min through the charcoal tubes. The average breakthrough air volumes are 18.5 L for enflurane and 15.9 L for halothane. The average capacities of the tubes were 0.255 mg of enflurane and 0.252 mg of halothane. The data collected for one test is plotted in Figure 4.5.1. with the (%) of halothane and enflurane in the downstream air vs. the total time of the test.

Table 4.5.
Sampler Capacity and Air Volume Sampled at 5% Breakthrough

test	enflurane	halothane	enflurane	halothane
	mg	mg	L	L
1	0.270	0.286	19.8	17.6
2	0.241	0.248	17.5	15.8
3	0.226	0.231	15.4	15.9
4	0.282	0.241	21.1	14.4

4.6. Desorption efficiency

Desorption efficiencies were determined by injecting amounts of enflurane and halothane in solution onto charcoal tubes. The amounts were equivalent to 0.5, 1, and 2 times the target concentration. Also, one set of tubes was prepared at the detection limit of the analytical procedure. The desorption efficiencies for both analytes over the concentration range evaluated was virtually 100% (Tables 4.6.1. and 4.6.2., Figures 4.6.1. and 4.6.2.)

Table 4.6.1.
Desorption Efficiencies for Halothane

x target conc.	0.02×	0.5×	1×	2×
µg/sample	1.87	42.1	84.2	168
desorption	101	100	102	102
efficiency, %	101	90	99	101
	100	94	99	104
	104	97	102	102
	101	95	104	102
	104	96	104	102
	102	95	102	102
= 100

Table 4.6.2.
Desorption Efficiencies for Enflurane

x target conc.	0.025×	0.5×	1×	2×
µg/sample	3.04	37.9	75	152
desorption	104	108	110	103
efficiency, %	104	100	108	112
	102	104	108	106
	103	104	102	108
	101	110	110	103
	103	110	106	106
	103	107	107	106
= 103

4.7. Storage data

Thirty-six samples were collected from a dynamically generated atmosphere of approximately 1 ppm of both halothane and enflurane. Relative humidity in the system was maintained at approximately 80%. Ten liters of this atmosphere were collected at 0.1 L/min.

Six of the samples were desorbed and analyzed the same day as collected. Of the 30 remaining tubes 15 were stored at ambient temperatures and 15 were refrigerated at approximately (5°C). Every third day, 3 samples from the ambient set and 3 from the refrigerated set were desorbed and analyzed. The results as % recovered are presented below and plotted in Figures 4.7.1. - 4.7.4.

Table 4.7.1
Storage Tests for Halothane

storage time	% recovery
(days)	(refrigerated)			(ambient)
0	82	88	84	90	84	90
3	95	93	101	93	94	97
6	96	91	99	89	94	100
10	98	99	102	94	99	100
12	98	98	99	96	98	95
15	100	99	98	94	97	99

Table 4.7.2.
Storage Tests for Enflurane

storage time	% recovery
(days)	(refrigerated)			(ambient)
0	105	107	103	105	101	103
3	91	92	107	96	93	97
6	99	94	110	94	108	104
10	106	105	107	100	106	112
12	98	99	101	97	101	96
15	110	113	109	lost	96	107

Figure 4.1.1. Detection limits of the analytical procedure.

Figure 4.4.1. Calibration curve for enflurane.

Figure 4.4.2. Calibration curve for halothane.

Figure 4.5.1. Breakthrough curves.

Figure 4.6.1. Desorption efficiency for enflurane.

Figure 4.6.2. Desorption efficiency for halothane.

Figure 4.7.1. Ambient storage test for enflurane.

Figure 4.7.2. Refrigerated storage test for enflurane.

Figure 4.7.3. Ambient storage test for halothane.

Figure 4.7.4. Refrigerated storage test for halothane.

Figure 4.8. Chromatogram of halothane and enflurane at their respective target concentrations.

5. References

5.1. Nicholson, John A.; Sada, Tsuneo; Aldrete, J. Antonio, Anesthesia and Analgesia. 1975, 54(4), 449 - 454.

5.2. Mazur, J.F.; Podolak, G.E.; Esposito, G.G.; Rinehart, D.S.; Glenn, R.E. Am. Ind. Hyg. Assoc. J. 1980, 41(5), 317 - 321.

5.3. Knill-Jones, R.P.; Moir, D.D.; Rodrigues, L.V.; Spence, A.A. The Lancet. 1972, I, 1326 - 1328.

5.4. "Criteria for a Recommended Standard...Occupational Exposure to Waste Anesthetic Gases and Vapors". NIOSH. (U.S.) 1977, Publ. No. 77 - 140.

5.5. Stevens, Wendell C.; Eger, Edmond I.; White, Anne; Biava, Claude G.; Gibbons, Robert D.; Shargel, Richard. Canadian Anesthesiology Society Journal. 1977, 24(4), 479 - 490.

5.6. Stecher, Paul G. (Editor) "The Merck Index". 8th edition. Rahway: Merck and Company, Inc.