NAME: Aspartame

SYNONYM(s): Succinamic Acid, 3-Amino-N-(alpha-carboxyphenethyl)-N-methyl Ester; Canderel; L-Phenylalanine, N-L-alpha-Aspartyl, 1-Methyl Ester; Equal; NutraSweet

IMIS: R273

CAS: 22839-47-0

NIOSH: RTECS WM3407000; 79173

DESCRIPTION:

MW: 294.34
MOLFM: C14H18N2O5

PRIMARY SAMPLING/ANALYTICAL METHOD (SLC1):

MEDIA:
PTFE Filter (37 mm; 1 micron)
ANL SOLVENT: 2.062 gm of 1-heptanesulfonic acid sodium salt to 1.0 L of 3:2 acetonitrile : water, adjust pH to 3.0 with H3PO4
MAX V: 1200 L   MAX F: 3 L/min
ANL 1: High Pressure Liquid Chromatography; HPLC/UV
REF: 1 (NIOSH 5031)
CLASS: Partially Validated
ART: Versella, G. Bagnasco, G. Mangia, A. "Journal of Chromatography" 349 (1985) 83-89 ...Title: Ion-Pair HPLC Analysis of Aspartame and Related Products ...Collection: 1 micron 37 mm PTFE filter with backup pad 1000 L at 2.5 L/min ...Analytical: HPLC C18 RCM Eluent: 60% [2.062 g 1-heptanesulfonic acid sodium salt and 0.45 g monobasic potassium in 1 L of DI water] and 40% [2.062 g 1-heptanesulfonic acid sodium salt in 1 L of 3:2 acetonitrile:water]. Detector: UV at 220 nm Albrecht, William N. Burr, Gregory A. Neumeister, Charles E. "Applied Industrial Hygiene" Vol 4, No 9, 1989, p 217. ...Virtually the same as the above article for sampling and analytical.

COND: Column: C18 SMobilie Phase: 60% Eluent A 40% Eluent B check method for mix.