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Chemical Sampling Information (CSI)
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General Description

OSHA IMIS Code Number: D137

Chemical Abstracts Service (CAS) Registry Number: 16071-86-6

NIOSH Registry of Toxic Effects of Chemical Substances (RTECS) Identification Number: GL7375000; 26972

Health Factors

Carcinogenic Classification:

International Agency for Research on Cancer (IARC): Benzidine Based Dyes - Group 2A, probably carcinogenic to humans (Technical-Grade)

Monitoring Methods used by OSHA

Primary Laboratory Sampling/Analytical Method (SLC1):

  • sampling media: Glass Fiber Filter (37 mm) without backup pad
    maximum volume: 100 Liters
    maximum flow rate: 1.0 L/min
    analytical 1: High Performance Liquid Chromatography; HPLC/UV/VIS
    method reference: 2 (OSHA In-house file)
    method classification: Partially Validated

Conditions: Special solvent extraction; u Bondapak C18; solvent programming, Initial 20% B - 30 min. to 80% B, A = 0.25 g Na2HPO4, 1.7 g TBAP, 60% water, 40% methanol, B = 0.25 g Na2HPO4, 1.7 g TBAP, 25% water 74% methanol; 1 mL/min; UV det: 436,546; DL 4.6 ng/inj; C/P DBA

Wipe Sampling Method:

  • sampling media: Glass Fiber Filter

Bulk Method:

  • For any dye analysis, a bulk sample of the dye must be sent to the Salt Lake Technical Center. Limit the amount of bulk submitted to one gram or approx. one mL. Protect samples from light and heat.

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