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Chemical Sampling Information (CSI)
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General Description

Synonyms: Rhodamine B

OSHA IMIS Code Number: 0848

Chemical Abstracts Service (CAS) Registry Number: 81-88-9

NIOSH Registry of Toxic Effects of Chemical Substances (RTECS) Identification Number: BP3675000; 5972

Health Factors

Carcinogenic Classification:

International Agency for Research on Cancer (IARC): Group 3, not classifiable as to its carcinogenicity to humans (Rhodamine B)
Monitoring Methods used by OSHA

Primary Laboratory Sampling/Analytical Method (SLC1):

  • sampling media: Glass Fiber Filters (37 mm)
    analytical solvent: Methanol
    maximum volume: 240 Liters
    maximum flow rate: 1.0 L/min
    current analytical method: High Performance Liquid Chromatography; HPLC/UV/VIS/FL
    method reference: 2 (OSHA PV2072)
    method classification: Partially Validated

Conditions: Column: C18 (40 deg C) Mobile Phase: 85:15 Acetonitrile: 0.005M 1-Heptanesulfonic Acid(pH3.5) Detector Wavelength: UV: 556nm FL: Ex: 210nm Em: 550nm Detection Limit: 0.013ng/inj Extraction w/ mobile phase; C18 column; 50% methanol 50% water; 1 mL/min; UV det: 254, 280; C/P DBA

Bulk Method:

  • current analytical method: High performance liquid chromatography; HPLC/U
    method reference: 2 (OSHA In-house File)
    method classification: Not Validated
    note: For any dye analysis, a bulk sample of the dye must be sent to the Salt Lake Technical Center. Limit the amount of bulk submitted to one gram or one mL.

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