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Benzidine Based Dyes

General Description

  • OSHA IMIS Code Number: B128
  • Chemical Abstracts Service (CAS) Registry Number: 92-87-5

Exposure Limits

  • National Institute for Occupational Safety and Health (NIOSH) Recommended Exposure Limit (REL): Lowest Feasible Concentration Carcinogen

Health Factors

Monitoring Methods used by OSHA

Primary Laboratory Sampling/Analytical Method (SLC1):
  • Polytetrafluoroethylene Filter, 5 micron
  • maximum volume: 500 Liters
  • maximum flow rate: 3.0 L/min
  • current analytical method: High Performance Liquid Chromatography; HPLC/UL52
  • method reference: 1 (NIOSH 5013 (PDF))
  • method classification: Partially Validated
  • note: This method does not differentiate between different dyes. Protect samples from heat and light.
Wipe Sampling Method:
  • Glass Fiber Filter
Bulk Method:
  • For any dye analysis, a bulk sample of the dye must be sent to the Salt Lake Technical Center. Limit the amount of bulk submitted to one gram or approx. one mL. If possible include Material Safety Data Sheet and color index number of dye.

Column: C18 mobile phase: 60:40 Methanol: Phosphate Buffer detection limit: Direct Blue 6 - 0.94 µg/m3, Direct Brown 95 - 7.7 µg/m3, Direct Black 38 - 15 µg/m3

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