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Acid blue 9

General Description

  • Synonyms: Brilliant Blue FCF; C.I. Acid Blue 9, diammonium salt; C.I. Acid Blue 9, disodium salt
  • OSHA IMIS Code Number: A156
  • Chemical Abstracts Service (CAS) Registry Number: 2650-18-2; 3844-45-9
  • NIOSH Registry of Toxic Effects of Chemical Substances (RTECS) Identification Number: BQ4550000; 6690
  • Chemical Description and Physical Properties:
    • Widely used food dye. Reddish-violet powder or granules with metallic luster. Soluble in ethanol and water. Insoluble in vegetable oils.

Health Factors

Carcinogenic Classification:

Date Last Revised: 08/24/1992

Monitoring Methods used by OSHA

Primary Laboratory Sampling/Analytical Method (SLC1):
  • Glass Fiber Filter (37 mm)
  • analytical solvent: (1:1) Methanol/Water
  • maximum volume: 100 Liters
  • maximum flow rate: 1.0 L/min
  • current analytical method: High Performance Liquid Chromatography; HPLC/UV/VIS
  • method reference: OSHA Analytical Method (OSHA PV2129)
  • method classification: Partially Validated
Bulk Method:
  • For any dye analysis, a bulk sample of the dye must be sent to the SLCAL. Limit the amount of bulk submitted to one gram or approx. one mL.

50% methanol/50% water extraction; Zorbax ODS; 55% methanol 45% water; 0.005 M TBAP; 1 mL/min; UV/VIS 650; Retention time, 7.8 min; DL 0.83 ng/inj; C/P YC 1/82

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