Chemical Sampling Information
	N-Nitrosodibutylamine
Quick Links General Description Exposure Limits Health Factors Monitoring Search    [use word(s)/phrase ]   Table of Contents By Name A B C D E F G H I J K L M N O P Q R S T U V W X Y Z By CAS Number   General Description Synonyms: Butylamine, N-Nitrosodi; N-Butyl-N-nitroso-1-butamine; DBN; DBNA; Dibutylamine, N-Nitroso; Dibutylnitrosamine; Di-n-butylnitrosamine; N,N-Di-n-butylnitrosamine; NDBA; N-Nitroso-di-n-butylamine OSHA IMIS Code Number: 1944 Chemical Abstracts Service (CAS) Registry Number: 924-16-3 NIOSH, Registry of Toxic Effects (RTECS) Identification Number: EJ4025000 Chemical Description and Physical Properties: pale yellow oil with characteristic odor molecular formula: C8H18N2O molecular weight: 158.24 boiling point: 116°C/14 mm Hg Health Factors National Toxicology Program (NTP) carcinogenic classification: Reasonably Anticipated to be Human Carcinogen [222 KB PDF] International Agency for Research on Cancer (IARC) carcinogenic classification: Group 2B, possibly carcinogenic to humans (N-Nitrosodi-n-butylamine) Environmental Protection Agency (EPA) carcinogenic classification: Group B2, Probable human carcinogen (N-Nitrosodi-n-butylamine) Potential symptoms: Irritation of eyes, skin; in animals: liver damage; [potential occupational carcinogen] Health Effects: Animal carcinogen (urinary bladder and multiple other sites) (HE2). Affected organs: Urinary bladder, esophagus, liver Notes: N-Nitrosodibutylamine is an OSHA Select Carcinogen. OSHA does not have a PEL for this chemical. Two major metabolites of this chemical, N-nitroso-n-butyl-N-(4-hydroxybutyl)amine and N-nitroso-n-butyl-N-(3-carboxypropyl) amine also cause bladder cancer in animals. Incubation of N-nitrosodibutylamine at a high concentration (4 mM) with human liver microsomes resulted in rates of production of the four metabolites, N-nitroso-n-butyl-N-(4-hydroxybutyl)amine N-nitroso-n-butyl-N-(3-hydroxybutyl)amine N-nitroso-n-butyl-N-(2-hydroxybutyl)amine and butyraldehyde in the approximate ratios of 1:13:4:1, respectively. N-Nitrosodibutylamine has been found in gastric juice and may be found in some foods and rubber products exposed to nitrite during production or utilization. Date Last Revised: 06/06/2006 Literature Basis: U.S. EPA Integrated Risk Information System: N-Nitroso-di-n-butylamine (CASRN 924-16-3). No Author: N-Nitrosodi-n-Butylamine, CAS No. 924-16-3. Report on Carcinogens, Eleventh Edition; U.S. Department of Health and Human Services, Public Health Service, National Toxicology Program, 2005. [222 KB PDF] Bellec, G., Dréano, Y., Bail, J.-P., Ménez, J.-F. and Berthou, F.: Cytochrome P450 hydroxylation of carbon atoms of the alkyl chain of symmetrical N-nitrosodialkylamines by human liver microsomes. Mutat. Res. 377(2): 199-209, 1997. Dallinga, J.W., et al.: Volatile N-nitrosamines in gastric juice of patients with various conditions of the gastrointestinal tract determined by gas chromatography-mass spectrometry and related to intragastric pH and nitrate and nitrite levels. Cancer Lett. 124(2): 119-125, 1998. Fiddler, W., Pensabene, J., Sphon, J. and Andrzejewski. D.: Nitrosamines in rubber bands used for orthodontic purposes. Food Chem. Toxicol. 30(4): 325-326, 1992. Heenan, M.P., Nacey, J.N., Delahunt, B., Ferguson, A.F. and Dickson, S.J.: Volatile N-nitrosamines in urinary catheters. Br. J. Urol. 63(1): 72-75, 1989. Pohanish, R.P. (editor): N-Nitrosodi-n-Butylamine. In, Sittig’s Handbook of Toxic and Hazardous Chemicals and Carcinogens, Fourth Ed., Vol. 2. Norwich, NY: Noyes Publications, William Andrew Publishing, 2002, pp. 1728-1730. Monitoring Methods used by OSHA Laboratory Sampling/Analytical Method: sampling media: ThermoSorb/N Tube analytical solvent: (75:25) Dichloromethane:Methanol maximum volume: 75 Liters   maximum flow rate: 1.0 L/min current analytical method: Gas Chromatography; GC/TEA method reference: OSHA Analytical Method (OSHA 27) method classification: Fully Validated note: OSHA personnel may obtain ThermoSorb/N Tube from SLTC. Store samples in freezer. Bulk method: Limit the amount of bulk submitted to one gram or one mL. All Trademarks are the property of their respective owners. Accessibility Assistance: Contact the OSHA Directorate of Science, Technology and Medicine at 202-693-2300 for assistance accessing PDF materials.