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Chemical Sampling Information (CSI)
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General Description

OSHA IMIS Code Number: 1012

Chemical Abstracts Service (CAS) Registry Number: 1937-37-7

NIOSH Registry of Toxic Effects of Chemical Substances (RTECS) Identification Number: QJ6160000; 50640

Health Factors

Carcinogenic Classification:

International Agency for Research on Cancer (IARC): Benzidine Based Dyes - Group 1, carcinogenic to humans [410 KB PDF, 8 pages]

National Toxicology Program (NTP): Known to be human carcinogen [169 KB PDF, 4 pages]
Monitoring Methods used by OSHA

Primary Laboratory Sampling/Analytical Method (SLC1):

  • sampling media: Glass Fiber Filter (37 mm) without a backup pad
    maximum volume: 100 Liters
    maximum flow rate: 1.0 L/min
    current analytical method: High Performance Liquid Chromatography; HPLC/UV/VIS
    method reference: 2 (OSHA In-House File)
    method classification: Partially Validated
    note: Ship with dry ice. Protect samples from light and heat.

Conditions: Special solvent extraction; u Bondapak C18; 20% B - curve 8 - 30 min to 80% B, A = 0.25 g Na2HPO4, 600 mL water, 400 mL methanol 1.7 g of TBAP, B = 0.25 g Na2HPO4, 250 mL H20, 750 mL methanol, 1.7 g TBAP; 1 mL/min; UV 546, 436; DL = 1.1 ug/sample; C/P DBA

Wipe Sampling Method:

  • sampling media: Glass Fiber Filter

Bulk Method:

  • For any dye analysis, a bulk sample of the dye must be sent to the Salt Lake Technical Center. Limit the amount of bulk submitted to one gram or approx. one mL.

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